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Fatty acids from lipids of marine organisms: Molecular biodiversity, roles as biomarkers, biologically active compounds, and economical aspects ArchiMer
Berge, Jean-pascal; Barnathan, Gilles.
Because of their characteristic living environments, marine organisms produce a variety of lipids. Fatty acids constitute the essential part of triglycerides and wax esters, which are the major components of fats and oils. Nevertheless, phospholipids and glycolipids have considerable importance and will be taken into account, especially the latter compounds that excite increasing interest regarding their promising biological activities. Thus, in addition to the major polyunsaturated fatty acids (PUFA) such as eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids, a great number of various fatty acids occur in marine organisms, e.g. saturated, mono- and diunsaturated, branched, halogenated, hydroxylated, methoxylated, non-methylene-interrupted. Various...
Tipo: Text Palavras-chave: Nutrition; Biomarkers; Marine organisms; Fatty acids; Lipids.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-937.pdf
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Gas chromatographic behavior of fatty acid derivatives for mass spectrometry on low-polarity capillary columns ArchiMer
Dubois, Nolwenn; Barnathan, Gilles; Gouygou, Jean-paul; Berge, Jean-pascal.
The gas chromatographic properties of four derivatives of fatty acids (FA), namely fatty acid methyl esters, picolinyl esters, N-acyl pyrrolidides and 4,4-dimethyloxazoline derivatives, which contain various structural features (double bonds, branching, hydroxyl group) in their acyl chains have been compared on a low-polarity capillary column with a mass spectrometer as detector. Temperature programming rates yielding the highest resolution were optimized for each derivative by means of computer-assisted column temperature optimization software. Indeed, the Drylab software represents a valuable assistance for estimating the optimum analysis conditions. Time and efforts required for such method development can greatly be reduced. Different parameters...
Tipo: Text Palavras-chave: Picolinyl esters N Acyl pyrrolidides Methyl esters GC MS Fatty acid derivatives 4; 4 Dimethyloxazoline.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6846.pdf
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Lipid content and fatty acid composition of a marine-derived Trichoderma longibrachiatum strain cultured by agar surface and submerged fermentations ArchiMer
Ruiz, Nicolas; Dubois, Nolwenn; Wielgosz Collin, Gaetane; Du Pont, Thibaut; Berge, Jean-pascal; Pouchus, Yves-francois; Barnathan, Gilles.
A marine-related Trichoderma longibrachiatum strain isolated from mussels in a farming shellfish area was investigated for total lipid (TL) production, total lipid fatty acids (TLFA), and phospholipid fatty acids (PLFA). Fungal biomass was produced from two different marine-like culture ways, on agar surface and submerged fermentations (ASF and SmF, respectively), allowing useful comparisons. ASF produced a rather higher biomass amount than SmF with similar TL content. All fatty acids (FA), identified by gas chromatography-mass spectrometry (GC-MS), were ranged from 14 to 30 carbon atoms. Similar FA compositions were found in TL and phospholipids (PL) from biomasses obtained by both fermentation systems, including oleic (up to 15.3% of total FA mixture for...
Tipo: Text Palavras-chave: Conjugated fatty acids; Fatty acids; Lipids; Submerged fermentation; Agar surface fermentation; Trichoderma longibrachiatum.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-4199.pdf
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Optimization of hydrolysis of sardine (Sardina pilchardus) heads with Protamex: enhancement of lipid and phospholipid extraction ArchiMer
Dumay, Justine; Allery, Marion; Donnay-moreno, Claire; Barnathan, Gilles; Jaouen, Pascal; Carbonneau, Marie Elise; Berge, Jean-pascal.
BACKGROUND: Fish by-products are not considered as valuable raw materials even if they usually contain valuable components such as lipids. Fish lipids are well known for their nutritional potential and health effects but their extraction remains problematic due to the use of organic solvents. Enzymatic hydrolysis such as the proteolysis of tissues can lead to lipid extraction. RESULTS: Hydrolysis of sardine heads by Protamex was studied (temperature, hydrolysis time and enzyme-substrate ratio) using response surface methodology in order to obtain the highest release of lipids and particularly phospholipids. No relation between the degree of hydrolysis and lipid recovery were depicted; however, optimum conditions for both the release of lipids and...
Tipo: Text Palavras-chave: Response surface methodology; Proteolysis; Sardine; By product; Fatty acids; Phospholipids.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6651.pdf
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Recovery of valuable soluble compounds from washing waters generated during small fatty pelagic surimi processing by membrane processes ArchiMer
Dumay, Justine; Radier, Severine; Barnathan, Gilles; Berge, Jean-pascal; Jaouen, Pascal.
This work focuses on the treatment of washing waters coming from surimi manufacturing using ultrafiltration technology at a laboratory scale. Four membrane materials (poly-ether sulfone, polyacrilonytrile, poly vinylidene fluoride and regenerated cellulose) and 5 Molecular Weight Cut-Off (from 3 to 100 kDa) have been studied at bench laboratory scale using the pilot Rayflow (R) 100, commercialised by Rhodia Orelis. The investigation deals with the ability for membranes to offer a high retention of biochemical compounds (proteins and lipids). Results obtained during adsorption tests showed that the regenerated cellulose material seems to be the most appropriate with regards to pore size reduction due to the protein-adsorption. During the ultrafiltration of...
Tipo: Text Palavras-chave: Fish oil; Regenerated cellulose; Surimi wastewater; Enzymatic hydrolysis; Ultrafiltration.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4359.pdf
Registros recuperados: 5
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